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Induction of apoptosis was detected by Propidium Iodide (PI, 1 g/mL) (Sigma Aldrich) and Annexin-V-APC (Immunotools) staining, followed by flow cytometry analysis, using a BD FACSCantoII flow cytometer

Zheng C, Yang Q, Cao J, Xie N, Liu K, Shou P, et al

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Being a prototype of so-called non-communicable diseases, the increasing prevalence of NAFLD, but also obesity, is regarded as closely related to changes associated with modern-day lifestyle including increased calorie intake, reduced physical activity, and sedentary behavior [2] that result in a mismatch between a decreased energy expenditure and an increased energy intake [3,4]