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After a washout period of at least 10 days, all the subjects were switched to receive the alternate coffee procedure

This diet is rich in vegetables, fruits, whole grains, legumes, fish, olive oil, and nuts, and has been shown to enhance sperm concentration, motility, morphology, and total count (Karayiannis et al., 2018

Constructs for cell experiments Constructs used in this study for cell culture experiments were obtained from GeneArt (Regensburg, Germany) in a pBUDCE4.1 vector optimized for mammalian expression for SLC constructs

Increased Receptor Affinity (Hypothesized): Some research suggests that the lack of the N-terminal tripeptide actually enhances its binding affinity to the IGF-1R in damaged or targeted cells, leading to a strong, immediate growth signal that promotes satellite cell recruitment, repair, and growth