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Description
In 3T3-L1 adipocyte cell models, 5-Amino-1MQ at 30 M concentration produced significant reductions in intracellular 1-MNA levels, confirming target engagement

Western blot Cells were harvested and proteins were extracted using cell lysis buffer supplemented with 0.3% phenylmethylsulfonyl fluoride and proteinase and phosphatase inhibitors

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