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Such behavioral deficits in A53T mice were accompanied by the presence of elevated -synuclein monomers and aggregates (Supplemental Figure 1J) and a remarkable loss of dopaminergic neurons in the substantia nigra, as indicated by tyrosine hydroxylase (TH) staining (Figure 1C)

Samples were evaporated and reconstituted in methanol prior MS analysis

In addition, raffinose increased peroxidation in the breast meat after slaughter, and this could negatively affect the quality and shelf life of the meat (78)

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