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Other commercial antibodies included: Normal rabbit IgG (Cell Signaling-2729S), Normal mouse IgG (Cell Signaling-5415S), Goat anti-mouse IgG1, Alexa Fluor 488 (Invitrogen-A21121), Goat anti-mouse IgG2b, Alexa Fluor 555 (Invitrogen-A21147), Goat anti-mouse IgM, Alexa Fluor 647 (Invitrogen-A21238), Peroxidase AffiniPure Goat antimouse IgG (Jackson ImmunoResearch-115-035-146), Peroxidase AffiniPure Goat antirabbit IgG Jackson ImmunoResearch (111-035-144) and Alexa Fluor 488-conjugated anti-rabbit (Invitrogen-A21206)