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Description
Reagents Dulbeccos Modified Eagle Medium (DMEM) including 4.5g/L glucose, L-glutamine, and sodium pyruvate was purchased from Corning (Mediatech, Inc., Manassas, VA) to culture mammalian cells
This helps to regulate inflammation levels, reduce oxidative stress, and promote cell regeneration and healing
It has virtually no direct blood supply
The samples were divided into two parts and stored in either anticoagulant (EDTA-K2) or non-anticoagulant tubes
