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then, untargeted metabolomics analysis was performed using a quantitative, automatic, and high-throughput LCMS approach to simultaneously determine 324 metabolites belonging to different important primary metabolic pathways, such as glycolysis, aerobic respiration, TCA cycle, fatty acid oxidation (FAO), and gluconeogenesis [20]

Cells were seeded in 96-well plates (5 10 3 cells/well) and incubated overnight at 37C

Secondly, it also discusses current applications of biologic therapy, herbal therapy, microecologics, and fecal microbiota transplantation (FMT), along with their prospects and challenges