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Gut microbiota modulates alcohol withdrawal-induced anxiety in mice

There remains a need to systematically elucidate the immune pathogenesis of extrapulmonary complications in prospective cohort studies, identify specific early diagnostic biomarkers, evaluate the efficacy and safety of different immunomodulators in pediatric patients with specific mechanistic phenotypes, and establish long-term follow-up data to clarify the impact of various immune interventions on patient long-term outcomes

Western blot analysis Cells were washed twice with cold PBS and lysed in RIPA buffer (R0278, Sigma-Aldrich), followed by vortexing and incubation on ice for 30 min