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4.11 Total proteomics and phosphoproteomics analysis by liquid chromatography combined with tandem mass spectrometry Be(2)-C cells were cultured in triplicates in 10 cm dishes and treated the following day either with MitoQ IC50, DPI IC50, Vin IC50, (M+D)/2, (M+D+V)/3, or vehicle for 24 h and washed once with PBS

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By preventing nicotinamide methylation, the compound increases intracellular NAD+ availability, which in turn activates sirtuins and other NAD+-dependent enzymes critical for metabolic homeostasis
