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Melatonin: from basic research to cancer treatment clinics

For the construction of the metE mutant, the AES -which contained a Kan R cassette and the GFP gene optimized for its expression in mycobacteria (eGFP) (Supplementary Note 1)- was synthesized by GenScript

Bacterial suspensions for infection were plated in solid agar medium to confirm the CFUs used for in vivo challenges

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