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We also investigate their preclinical and clinical insights for primary liver cancer intervention, and explore the significance of using Wnt/-catenin mutations as a biomarker to predict resistance in immunotherapy
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For analysis of miRNAs, cDNA was synthesized from 10 ng of DNase Itreated total RNA using the TaqMan microRNA reverse transcription kit (Applied Biosystems PN4366596) with RT primers from TaqMan sets for miR18a (Cat #002422), and snoRNA202 (Cat #4427975) as endogenous control
