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In a mechanistic study with different types of tumour cell lines, the silencing of Grx1 activated the p53 signalling pathway, causing the cell cycle to stop in G1 causing senescence [188], which supported the idea that antioxidant systems, including Grx1, get upregulated in tumour cells as survival mechanism to counteract the high amount of ROS due to the changes in metabolism

Li JY, Zhang Q, Yang XY, Wu HM, Lin RL, He HB

[0440] In certain embodiments to maintain disulfide bonds, cysteine residues can be engineered into proteins