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Description
The remainder of the supernatant was transferred into a microcentrifuge tube containing the GFP-Trap beads as the immunoprecipitation sample and was then placed on a rotator for 45 min at 4 C
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A guide RNA determines the sequence specificity of any RNA silencing mechanism, however, the particular type of silencing is determined by AGO protein characteristics such as interacting proteins, ribonuclease activity, and subcellular localization
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