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Unexpected tautomeric equilibria of the carbanion-enamine intermediate in pyruvate oxidase highlight unrecognized chemical versatility of thiamin

doi: 10.3389/fncel.2025.1593963 Received 15 March 2025 Accepted 27 June 2025 Published 22 July 2025 Volume 19 - 2025 Edited by Alessandro Tozzi, University of Perugia, Italy Reviewed by Antonio Martnez-Ruiz, Princess University Hospital, Spain Mingzi Zhang, University of Southern California, United States Walter Moos, University of California, San Francisco, United States Updates Copyright 2025 Huang, Zhang, Chen, Wang and Zhou

The continuous presence of glutathione throughout the day allows for diminishing the level of toxin or oxidation stress that has compromised the function of the methionine pathway

BPh not only facilitated desorption and ionization but also functioned as a derivatizing agent, performing laser-driven PB reactions to functionalize unsaturated PLs without the need for additional equipment and generating PB product ions for various PL classes