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Monomeric fractions of hFcRn-His were isolated by SEC using a Superdex 200 Increase 10/300 column (Cytiva Life Sciences) with an KTA Avant 25 (Cytiva Life Sciences)
Figure 1: Folate metabolism starts with polyglutamate folates (a form of Vitamin B9), which are broken down by the enzyme dihydrofolate reductase (DHFR) into dihydrofolate and then tetrahydrofolate (THF)
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