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Areas requiring further investigation include: Tissue-specific dose optimisation: While 10-20 g/kg appears broadly effective across various tissues, whether specific tissues (cartilage versus muscle versus nerve) respond optimally to different doses has not been systematically explored

Adequate folate intake supports DNA synthesis, methylation and normal cell division, and sufficient folate status before the development of pre-cancerous changes appears to reduce disease risk

Gene-level modulation: Pickart & Margolina 2018 documented activity across ~4,000 human genes, with fibroblast expression patterns shifting closer to younger cells

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