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Loss of hepatocellular volume and retrieval of Mrp2 from the canalicular membrane occur concomitantly after addition of tBOOH71,127 or chloro-2,4-dinitrobenzene71 to isolated perfused rat livers, and hepatocyte shrinkage induced by hyperosmotic exposure in this experimental model triggers a similar retrieval.128 However, under these hyperosmotic conditions, distribution of the tight-junctional-associated protein, ZO-1, remains unaffected.128 3.3

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Therefore, the functions of these proteins or enzymes are lost or altered [39]

This may not be necessary every time you have injections, but regular testing is helpful to track your progress