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Ten microlitres of the digested tissue medium was then mixed with 100 l of the DMMB dye
You will learn exact microgram amounts, precise timing windows, reconstitution ratios that preserve potency, cycling strategies that prevent tolerance, and troubleshooting protocols for when things go wrong
We further demonstrate that a T291A/T292A double variant of ArfGAP1 induces an intermediate level of Golgi fragmentation relative to WT and 3A ArfGAP1 (Supplementary Figure S1)
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