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The agarose beads were washed three times with 1 ml of lysis buffer and then washed twice with reaction buffer (20 mM HEPES, pH 7.4

From a basic science standpoint, acetylcysteine neutralizes NAPQI in a 1:1 molar ratio, so the dose of acetylcysteine should be scaled up in proportion to the amount of acetaminophen

In this study, as described in detail hereinbelow, both the placebo (n=19) and the intervention (n=21) groups demonstrated improvements from baseline to follow-up in some areas of autism behavior