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doi: 10.1016/j.smim.2020.101410

Briefly, pellets from the bacterial culture were resuspended in periplasmic extraction buffer (100 mM Tris/Cl pH 8.0, 0.5 M sucrose, 1 mM EDTA), incubated for 30 min at 4 C, and centrifuged repeatedly to remove the spheroplasts

Even in healthy women between 3060 years of age, telogen effluvium can occur WITHOUT a trigger

However, NAC as an antiviral drug is quite controversial
