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Feature plots visualization revealed a reciprocal expression pattern of OLFM4 and TACSTD2 , established markers of the fetal intestinal state 15 , indicating that Matrigel organoids predominantly exhibit a mature, adult epithelial phenotype, whereas collagen organoids acquire a revSC phenotype consistent with fetal reversion (Fig

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Singlet oxygen ( 1 O 2 ) Thermo #S36002 (in lysates fluorometry)

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