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Simultaneously, angiogenesis-related factors induce macrophage polarization toward the M2 phenotype, thereby leading to the development of an immunosuppressive tumor microenvironment (TME)

This VDF cocktail induced high expression of key hepatic markers such as AFP, HNF4, and Albumin (ALB), with an efficiency of 95.5% (HNF4a+/AFP+ positive cells), closely matching that of the growth factor-based control