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Since differences in background staining intensity and protein half-life hampered a direct comparison of dsRNA and NS3 levels, we aimed to validate these results by quantifying dsRNA in total RNA isolated from neoJFH1 wt and D1467G stable cell lines using a commercially available split-nanoluciferase-based assay

GHK-Cu is the most well-characterized of the three in terms of extracellular matrix effects

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After nano LC separation, eluting peptides were ionized utilizing a CaptiveSpray source (Bruker Daltonics, Bremen, Germany) in positive mode, setting the capillary voltage to 4.5 kV, the nebulizer to 0.4 bar, and the dry gas flowrate and temperature to 3 L/min and 180C, respectively