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By mapping the cascade from zinc imbalance to molecular dysfunction, organelle stress and disease expression, the framework provides an early overview that anchors the detailed sections that follow and highlights mechanistic points of intervention with potential relevance for future therapeutic modulation 18,19,20,21

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In vitro, human renal proximal tubular epithelial cells (HK-2) were exposed to calcium oxalate monohydrate crystals (1.5 mM), or cells were pretreated with PS-NPs (100 nm, 0.1 mg/mL) for 24 h, followed by the addition of 1.5 mM CaOx for co-treatment