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Moreover, in SH-SY5Y neuroblastoma cells, A42 induced thinning of rafts that altered their physicochemical properties and membrane perturbations only in more fluid, cholesterol-depleted membranes, indicating a protective effect of cholesterol against A42 toxicity (Cecchi et al., 2009)
doi: 10.1039/D5FB00122F 63
That is worth repeating

Many researchers who plan to escalate beyond 4mg switch to a new vial with 1mL reconstitution at that point rather than splitting draws