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The ALT positive U2OS cells were included as a positive reference control, and C-circles were presented as percentages relative to the U2OS cell line (Henson et al

No dose-limiting toxicities were observed at any tested dose

Quantitative Real-Time PCR (qRT-PCR) was used to measure mRNA expression of SASP factors (IL-1, IL-6, TNF-, TGF-) in TM3 cells, normalizing data to GAPDH

However, average total scores on the GSQS across the seven nights are also correlated with scores on the Emotional instability subscale of the Big Five Inventory personality scale (r = 0.278, p = 10 6 ), the Neuroticism-Anxiety subscale of the Zuckerman-Kuhlman Personality Questionnaire (r = 0.245, p = 10 4 ), weekly average negative emotional ratings of their days on the Positive and Negative Affect Scale (r = 0.274, p = 10 6 ), and the mean subjective ratings of their days as Happy as opposed to Sad on a Likert scale (r = 0.189, p = 0.002)