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PC quantification was performed in an enzyme-linked fluorescent assay (ELFA) using VIDAS Protein C assay

Their findings showed that BDNF protein expression in rat embryonic NSCs transfected with the human BDNF gene (BDNF-NSCs) was upregulated, while neurite outgrowth in ganglion neurons were simulated, suggesting that BDNF increased neurogenesis in vitro

Thus pre-treatment with L-arginine counteracted the antidepressant-like effect of imipramine, venlafaxine and bupropion (Krass et al., 2011), while inhibition of nitric oxide levels within the hippocampus can induce antidepressant like effects (Joca and Guimaracs, 2006)

Overcoming these challenges often relies on multilevel optimization