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The lysate with LLO was loaded onto 30 ml Ni-NTA Superflow resin (QUIAGEN, Germany) packed in a XK 16/200 column (GE Healthcare, USA), washed with wash buffer (20 mM MES/NaOH pH 6.5, 150 mM NaCl), and eluted with an imidazole gradient in 20 mM MES/NaOH pH 6.5, 150 mM NaCl, with 500 mM imidazole as the final concentration

[84] [86] Dextroamphetamine, the more dopaminergic enantiomer of amphetamine, is particularly effective at promoting wakefulness because dopamine release has the greatest influence on cortical activation and cognitive arousal, relative to other monoamines

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Para algunas personas con restricciones dietticas especficas, la suplementacin puede ser una opcin para cubrir sus necesidades