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This structure critical for biological activity

Raffaele, Stefano, Marta Lombardi, Claudia Verderio, and Marta Fumagalli

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Doxorubicin hydrochloride (Dox), Dowex, selenium powder (Se 200 mesh), Sodium borohydride (NaBH 4 ), 10-Bromodecanoic acid, magnesium sulfate (MgSO 4 ), Hydrogen peroxide (H 2 O 2 ) solution 30% (w/w), Dimethylformamide (DMF), dimethyl sulfoxide (DMSO), Diethyl ether, Ethanol 98%, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were obtained from Sigma-Aldrich (Taufkirchen, Germany), C26 cell line (murine colon carcinoma cells) and NIH-3T3 cell line (mouse embryonic fibroblast cells) were purchased from Cell Lines Service (Eppelheim, Germany), RPMI 1640 and DMEM culture media and fetal bovine serum (FBS) were purchased from Gibco (Carlsbad, CA)