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Step 1: Reconstitute the Peptide in the Original Vial Peptides should always be reconstituted first in their original vial using sterile bacteriostatic water, following standard laboratory technique
Dynorphin released from striatal inputs could be an autofeedback mechanism, heterosynaptic (targeting pallidal input), or directly inhibit EPN neurons (Ogura and Kita, 2002)
Tissues were homogenized and lysed in NETN buffer (20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM EDTA and 0.5% Nonidet P-40) supplemented with 50 mM -glycerophosphate, 5 mM NaF and a protease inhibitor cocktail (Roche)
Wichmann, A
