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The amplification of miRNA cDNAs was quantified in real-time SYBR Green RT-qPCR reactions according to the recommended two-step cycling procedure (preincubation/activation at 95 C for 2 min followed by 40 cycles of 95 C for 5 s, 60 C for the 30 s)

Interestingly, application of 6-mercaptopurin accompanied by Zn 2+ sulfate used to treat acute lymphoblastic leukemia did not produce this effect, but notably the application was over a significantly shorter time period (40)