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Standard research protocol structure: Loading phase (week 1) : 300mcg daily, initiated 35 days post-injury or surgical repair Active phase (weeks 26) : 300mcg daily, continued through hard callus formation Taper phase (optional, weeks 78) : 150mcg daily or every other day if extending beyond six weeks Daily administration maintains more stable plasma levels than alternate-day or pulsed protocols

Selective NNMT inhibitors (including 5-Amino-1MQ) lower 1-MNA, increase NAD and SAM, suppress adipocyte lipogenesis, and reverse HFD-induced obesity in mice [1]

Animal products contain the most bioavailable b12

For context (weekly totals): IM up to 3/week: 50 mg 3 = 150 mg/week