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Maintenance Therapy : For ongoing maintenance, a monthly injection of 1 mg (1000 g) is generally sufficient to maintain adequate vitamin B12 levels in most adults

P2 was suspended in 10 vol of 0.32 M sucrose and 10 ml of the solution layered on a 0.8 and 1.2 M two-step discontinuous gradient, which was centrifuged at 61,000 x g for 2 h to separate the synaptosomes from the mitochondria (which forms the pellet)

Training mice at glucocorticoid peaks resulted in higher rate of spine formation, whereas glucocorticoid troughs following training was necessary for stabilization of spines formed during training and long-term memory retention (Liston et al., 2013)