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Description
Time traces were recorded in Corning 96-well non-binding surface plates using a Varioskan (ThermoFisher, Waltham, MA) plate reader using a ecc of 490 nm and a em of 510 nm at 37 C, shaking the samples for 10 s before each read
and (2) nicotinamide a NAD+ precursor is diverted away from the NAD+ synthesis pathway into the methylation pathway, reducing cellular NAD+ levels
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Microchim Acta 176, 122 (2012)
