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Sigma) for 4 hours at room temperature, washed in 8% glucose (in 0.12 M phosphate buffer and 0.02 mM CaCl 2 , pH 7.27.5) and postfixed in 2% OsO 4 (in 0.12 M phosphate buffer and 0.02 mM CaCl 2 , pH 7.4

Research has increasingly highlighted the importance of non-adherent culture conditions and low oxygen environments for successful in vitro cultivation of CTCs [126]

in the past, the latter two have led to highprofile tragic incidents with numerous deaths (replacement of glycerine with ethylene glycol in cough syrup for children)

Springer Singapore Rajagopal S, Ramachandran S, Sundararaman G, Gadde S, Editors V (2022) Role of nutrients in neurological disorders Aishworiya R, Valica T, Hagerman R, Restrepo B (2022) An update on psychopharmacological treatment of autism spectrum disorder