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Composed of three amino acidsglutamate, cysteine, and glycineit is the most abundant non-protein thiol in mammalian cells, where it exists in two primary states: the reduced form (GSH) and the oxidized form, glutathione disulfide (GSSG)

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Brain glutamine synthetase, localized in all cells of the astroglial family (Anlauf and Derouiche 2013), increases after the ischemic event (Petito et al

6d) (Tsikas et al., 1999) while another method uses a biotinylated fluorescent label in a method called the biotin-switch (Gladwin et al., 2006)