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Description
Reverse transcription was applied at 50 C for 30 min, a primary denaturation step was done at 95 C for 5 min, followed by 35 cycles of 94 C for 30 s., annealing (55 C for 40 s for bovine viral diarrhea virus)

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After ingestion, MK-677 increases somatotrophe secretion of GH via signalling the pituitary gland to secrete a sufficient amount of growth hormone [R, R]

The actual procedure itself took less than 30 minutes
