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As a result, the stratum corneum dehydrates, loses its flexibility, and forms small cracks, which make the surface of the skin dull, rough, and scaly
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All mRNA levels were determined by real-time quantitative polymerase chain reaction (qPCR) using a Thermo Fisher Quant Studio 3 Real-Time PCR Instrument (Thermo Fisher Scientific, Waltham, MA, USA) and SYBR qPCR mix (Toyobo Co., Ltd., Osaka, Japan)
doi: 10.1111/j.1532-5415.2010.03232.x [DOI] [PubMed] [Google Scholar] 101.Langan-Evans C, Hearris MA, Gallagher C, et al
