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After removing the culture medium and washing twice with PBS, cells were incubated with 0.1 M of either free GFP (Abcam plc, Cambridge, UK) or GFP-NI for 12 h

Studies in Crat knockout mice demonstrated that CrAT deficiency leads to abnormal fuel selection, which results in a perturbation of glucose homeostasis and suggest that deficits in CrAT activity might contribute to diet-induced metabolic inflexibility by exacerbating the Randle glucose-fatty acid cycle 66

Although recurrent and metastatic tumors disappeared or diminished in size by radiation, tumors increased in size thereafter, despite additional adjuvant chemotherapy