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Key pathways include PI3K/Akt and MAPK, which promote fibroblast proliferation, collagen synthesis, and angiogenesis

Cells were seeded at a density of 1 10 6 cells/mL and were cultured for 24 hours (n = 11) or 72 hours (n = 19) in RPMI-1640 medium supplemented with 10% human serum (Sigma-Aldrich) and penicillin-streptomycin (100 U/g/mL)

In addition to the quantification of R6G, we made efforts to determine the concentration of dopamine and ibuprofen from the samples of the permeability experiments by liquid chromatography-tandem mass spectrometry