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The intrinsic factor then binds with B12, facilitating the uptake of B12 in the small intestines.[ref] Without intrinsic factor, you cant absorb B12 from animal proteins

doi: 10.1126/science.72.1855.76

Calibration of the instrument was achieved using Pierce LTQ ESI Positive (#88323

Expression of large amino acid transporter LAT1 in rat brain endothelium
