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Therefore, while metabolism-targeted combination therapies are being advanced, a systematic evaluation of their risk-benefit ratios is necessary to overcome the aforementioned limitations through rational design

J Cell Sci, 2023

Total protein extracts were obtained by homogenization of tissues using protein sample buffer [100 mM Tris-HCl (pH 6.8), 200 mM dithiothreitol, 4% sodium dodecyl sulfate (SDS), 0.2% bromphenol blue and 20% glycerol] and a classic protease inhibitor cocktail (Beyotime Institute of Biotechnology)

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