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Lets take a closer look at each criterion in more detail to better understand how we came to our conclusions

10% glycerol was added, and samples were loaded onto Novex TBE, 10% native polyacrylamide gels (Invitrogen), subsequently stained with SYBR Gold (Invitrogen) and scanned on a Typhoon FLA 9500 imager using its control software (v.1.1, Amersham, GE)

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