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Briefly, frozen tissue was powdered with a nitrogen-cooled mortar and pestle, and 50-70mg of tissue were homogenized in the absence (for GSH determination) or presence (for GSSG determination) of 1-methyl-2-vinylpyridinium trifluoromethanesulfonate to scavenge reduced thiols

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The model was able to predict total and unbound VPA plasma concentrations, along with concentrations of VPA metabolites (VPAglucuronide, 2enVPA and 4enVPA) for both single and multiple dosing