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The cells were collected by centrifugation at 2000 g for 5 min and washed in phosphate buffered saline (8 gL 1 NaCl, 0.2 gL 1 KCl, 1.44 gL 1 Na 2 PO 4 , 0.24 g L 1 KH 2 PO 4 , at pH 7.5)
12, 13, 14 Similarly, the class I PTC W1282X mutant exhibits compromised NBD1-NBD2 dimerization due to a partial deletion of NBD2, leading to folding defects and reduced activity (which would be proper of class II and III mutants)
10.18502/ijph.v48i6.2929 [PMC free article] [PubMed] [Google Scholar] 182.Izquierdo-Pulido M, Zern-Rugerio MF, Ibarra-Picn A, Diez-Hernndez M, Prez-Cano F, Cambras T
10.1161/CIRCIMAGING.112.974998 17
