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Description
In this work, we describe the generation and validation of a panel of nanobodies selective for the luminal domain of Syt1, which we optimized into a diverse array of probes collectively referred to as the NbLumSyt1 toolkit
This framework helps distinguish whether an individual has actual nutrient insufficiency, mitochondrial stress, or metabolic dysfunction that might benefit from component-level support versus lifestyle intervention alone
Precursor ion mass spectra were acquired at 120,000 resolution, scan range 375-1,500 and maximum injection time 50 ms
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