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Model building and refinement An initial model of Ycf1 was built using the SWISS-MODEL server 46 and PDB ID: 6JB1 as a template 47

Quantitative RT-PCR validation To further verify the RNA-seq results, we performed quantitative real-time PCR (qRT-PCR) analysis of 17 genes

In the QH2 form it enters Complex III to transfer electrons to cytochrome c and pump protons into the intermembrane space of the mitochondrion

As detected in mice and also in other species, the levels of glutamate and GABA in the hippocampus and cortex show an age-dependent decline (Duarte et al
