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It should be noted that the evidence supporting this mechanism mainly originates from a single research group, and its reproducibility across different OA models has not been fully validated

Cyclooxygenase-2 (COX-2), encoded by the PTGS2 gene, accelerates the metabolism of arachidonic acid and amplifies inflammation through the secretion of inflammatory signaling molecules [24]

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Laboratory Handling and Storage Protocols Lyophilized Powder Storage: Store at -20C to -80C in original sealed vials with desiccant Protect from light exposure (amber vials or aluminum foil wrapping) Maintain consistent low-temperature storage without freeze-thaw cycles Avoid moisture exposure