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[DOI] [PMC free article] [PubMed] [Google Scholar] 14.Zhang W.C., Shyh-Chang N., Yang H., Rai A., Umashankar S., Ma S., Soh B.S., Sun L.L., Tai B.C., Nga M.E., et al

The negative effects are mostly due to the injection process, which leads to a compromised shell and membrane of the egg, allowing for pathogenic bacteria and environmental factors affecting the egg and embryo

2.4 Sample preparation For LCQTOF-MS/MS analysis, 1 mg of powder or homogenized tablet was dissolved or extracted using 5 mL of a 1:1:1 (v/v/v) mixture of water/methanol/acetonitrile, assisted by ultrasonication for 10 min, and afterwards filtered by a Whatman 0.2 m pore size polytetrafluoroethylene (PTFE) filter medium (GE Healthcare, Chicago, IL, United States)

Taking it right alongside chai or coffee