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Substrate utilization in the cortical collecting duct is qualitatively similar to the thick ascending limb 17,18,19,23
10.1242/dev.00111
ROS and glutathione measurement For ROS measurement, T cells were incubated at 37 C for 20 min in PBS containing DCFHDA (1:1000, #S0033S, Beyotime, China) for cellular ROS detection or with Mito-SOX (1:200, #M36008, Invitrogen, USA) for mitochondrial ROS detection
